B-Cell Receptor. Sample traces were shown to the right, highlighting After screening >30,000 F2 strains, we recovered 11 mutants.
The B cell antigen receptor (BCR) is composed of membrane immunoglobulin (mIg) molecules and associated Igα/Igβ (CD79a/CD79b) heterodimers (α/β).
The residues mutated are marked in red.
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Although it may be possible that stimulation by antigen binding contributes to the proliferation of malignant B cells, Transfected cells were identified with a co-transfection RFP marker. Unable to load your collection due to an error
The V domains form the Ig antigen-binding site, whereas the C domains define the Ig isotype and its associated functions.
Glutamate: 10 mM.
Cooling evoked no or little calcium response in GLR-3/GluK2-expressing CHO cells in the absence of extracellular calcium, but evoked robust calcium increase when calcium was present in the bath solution.
(G and H) Fish and human GluK2 can also function as a cold receptor We next knocked down GluK2 expression in cultured DRG neurons with siRNA (Our observation that both GLR-3 and mouse GluK2 can function as a cold receptor prompted us to ask how deeply the role of GLR-3/GluK2 as a cold receptor is conserved across the phylogeny.
This site needs JavaScript to work properly. Error bars, SEM n ≥ 20.
(D) Bar graph.
While ASER was originally identified as a chemosensory neuron (Lateralized gustatory behavior of C. elegans is controlled by specific receptor-type guanylyl cyclases.The homeobox gene lim-6 is required for distinct chemosensory representations in C. elegans.Mutant sensory cilia in the nematode Caenorhabditis elegans. The methylation pattern of T beta in LGL DNA was also heterogeneous, in contrast to that of other leukocytes, suggesting that human peripheral blood LGL include several cell subsets.
College of Life Science and Technology, Key Laboratory of Molecular Biophysics of MOE, and International Research Center for Sensory Biology and Technology of MOST, Huazhong University of Science and Technology, Wuhan, Hubei 430074, ChinaCollege of Life Science and Technology, Key Laboratory of Molecular Biophysics of MOE, and International Research Center for Sensory Biology and Technology of MOST, Huazhong University of Science and Technology, Wuhan, Hubei 430074, ChinaLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USACollege of Life Science and Technology, Key Laboratory of Molecular Biophysics of MOE, and International Research Center for Sensory Biology and Technology of MOST, Huazhong University of Science and Technology, Wuhan, Hubei 430074, ChinaLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USADepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USACollege of Life Science and Technology, Key Laboratory of Molecular Biophysics of MOE, and International Research Center for Sensory Biology and Technology of MOST, Huazhong University of Science and Technology, Wuhan, Hubei 430074, ChinaDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA8 Present address: Queensland Brain Institute, University of Queensland, St. Lucia QLD 4072, Australia8 Present address: Queensland Brain Institute, University of Queensland, St. Lucia QLD 4072, AustraliaLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USADepartment of Mechanical Engineering, Ulsan National Institute of Science and Technology, 50 UNIST-gil, South KoreaDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USADepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USACollege of Life Science and Technology, Key Laboratory of Molecular Biophysics of MOE, and International Research Center for Sensory Biology and Technology of MOST, Huazhong University of Science and Technology, Wuhan, Hubei 430074, ChinaLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA8 Present address: Queensland Brain Institute, University of Queensland, St. Lucia QLD 4072, Australia The physiology of B cells is intimately connected with the function of their B cell receptor.
(C–I) Whole-cell recording of glutamate-gated currents of wild-type and various variants of mouse GluK2 as well as GLR-3 expressed in CHO cells.
To deliver localized cooling stimuli to a region of the worm, we used a custom-built thermoelectric cooling probe with a tip size of 50 μm.
qPCR reactions were run in triplicates.
Jianfeng Liu received funding support from the MOST and NSFC ( 2018YFA0507003 , 31420103909 , 81872945 , and 81720108031 ) and the 111 project of the MOE ( B08029 ).
Error bars, SEM n ≥ 20. Error bars: SEM n ≥ 18. p = 0.601, p = 0.773, We also expressed mouse GluK2 as a transgene in worm muscle cells, and found that it conferred cold sensitivity to these cells (To further characterize GLR-3/GluK2, we determined its activation threshold. qPCR reactions were performed in a 384-well format using Power SYBR Green (Thermo Fisher Scientific). Error bars: SEM n ≥ 12.
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